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It also helped to explain the important metabolic interrelationship among vitamin B, folic acid, and one-carbon metabolism.

On a personal note, it was during this time that I made the transition from a postdoctoral fellow to an independent scientist, and as I will discuss, these studies on vitamin B, directly or indirectly, provided the foundation for much of the later research I have been involved in.

Two independent pathways for DMB synthesis also exist (18).

Aerobic DMB synthesis occurs by fragmentation of a flavin cofactor and is catalyzed by the Blu B enzyme (6, 19, 27).

I am taking the liberty throughout this article to include some biographical information and personal thoughts since it is hard for me to reflect on my early years in science without providing some personal history.

I was raised in the Bronx, the middle of three children with a brother, Arthur, 5 years older, and a sister, Carol, 7 years younger.

The main difference between these pathways is in the participation of oxygen.

According to a reference in the , Warburg had been shortlisted in 1944 for a second Nobel Prize based on his work on flavoproteins, but he could not receive it due to the political situation in Nazi Germany; however, the authenticity of that report is doubtful..

In fact, a modification of that work that was introduced by Tishler was actually used for decades for the bulk production of the vitamin.

The “aerobic” pathway requires oxygen and has been characterized in depth in biosynthetic enzymes is restricted to anaerobic or microaerobic conditions (22, 30).

Nucleotide loop assembly is similar in both pathways (23, 30).




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